The Amazing Spider-Man, Vol.2 #541

Peter has learned the identity of the man who ordered the shot that put Aunt May in the hospital, and it's none other than Wilson Fisk... the Kingpin. Even in prison, Fisk has considerable influence over the criminal world in New York. Determined to make Fisk pay, Peter prepares for a confrontation in which he fully expects to kill the Kingpin. But before he can go, May's situation has not improved. The machines in the hospital are the only things keeping her alive, though there isn't any brain damage yet. Once, years before, a transfusion of Peter's blood saved her life. With nothing left to lose, Peter hopes that this desperate gambit may pay off once again.